mRNA堿基修飾檢測

  • 簡介
  • 分析種類
  • 結果展示

  • mRNA化學修飾的定量檢測是表觀轉錄組學研究所必不可少的環節。檢測不同處理條件下的表觀轉錄修飾變化將為下一步的研究提供重要線索。Aksomics(原2022世界杯竞猜app下载生物)的質譜檢測平台能夠為客戶提供全麵而準確的mRNA修飾譜。利用Agilent質譜儀,檢測35種修飾堿基在各個樣品中的修飾豐度。



    服務特色:

    ● 優化的前期處理與Agilent優質設備 

    ● 全麵而準確的堿基修飾分析


    分析流程:

    LC-MS mRNA修飾檢測服務流程圖

    圖釋:LC-MS mRNA修飾檢測服務流程圖。


  • Number Nucleoside Symbol Number Nucleoside Symbol
    01 3′-O-methyladenosine 3′-OMeA 27 3'-O-methyluridine 3'-OMeU
    02 2′-O-methylcytidine Cm 28 5-methyl-2-thiouridine m5s2U
    03 3-methylcytidine m3C 29 5-methoxyuridine mo5U
    04 5-methylcytidine m5C 30 pseudouridine Ψ
    05 N6-isopentenyladenosine i6A 31 2'-O-methylinosine Im
    06 5,2'-O-dimethylcytidine m5Cm 32 3-methyluridine m3U
    07 1-methyladenosine m1A 33 1-methylpseudouridine m1Ψ
    08 2-thiocytidine s2C 34 5-hydroxymethylcytidine hm5C
    09 N2,N2,7-trimethylguanosine m2,2,7G 35 5,2'-O-dimethyluridine m5Um
    10 N4-acetyl-2'-O-methylcytidine ac4Cm 36 N6-threonylcarbamoyladenosine t6a
    11 N6-methyladenosine m6A 37 2-methylthio-N6-threonylcarbamoyladenosine ms2t6A
    12 3'-O-methylcytidine 3′-OMeC 38 5-carboxymethyluridine cm5U
    13 2'-O-methyladenosine Am 39 5-methoxycarbonylmethyl-2-thiouridine mcm5s2U
    14 N2, N2-dimethylguanosine m22G 40 5-Methoxycarbonylmethyluridine mcm5U
    15 5'-O-methylthymidine 5′-OMeT 41 2-methylthio-N6-isopentenyladenosine ms2i6A
    16 2′-O-methyluridine Um 42 Peroxywybutosine o2Yw
    17 inosine I 43 5-taurinomethyl-2-thiouridine tm5s2U
    18 2′-O-methylguanosine Gm 44 5-oxyacetic acid uridine cmo5U
    19 1-methylguanosine m1G 45 5-carbamoylmethyuridine ncm5U
    20 7-methylguanosine m7G 46 Queuosine Q
    21 N2-methylguanosin m2G 47 5-taurinomethyluridine tm5U
    22 3'-O-methylinosine 3'-OMeI 48 5-formyl-2′-O-methylcytidine f5Cm
    23 2-thiouridine s2U 49 dihydrouridine D
    24 4-thiouridine s4U 50 5-formylcytidine f5c
    25 5-methyluridine m5U 51 wybutosine yW
    26 N4-acetylcytidine ac4C 52 5-methoxycarbonylmethyl-2'-o-methyluridine mcm5Um
  • 1. 修飾定量分析

    mRNA修飾數量分析圖

    圖釋:mRNA修飾數量分析圖。


    2. 總離子流程圖

    總離子流程圖

    圖釋:總離子流程圖